Reciprocating-flow ATP amplification system for increasing the number of amplification cycles
نویسندگان
چکیده
منابع مشابه
Reciprocating-flow ATP amplification system for increasing the number of amplification cycles.
We constructed a novel ATP amplification reactor using a reciprocating-flow system to increase the number of ATP amplification cycles without an increase in backpressure. We previously reported a continuous-flow ATP amplification system that effectively and quantitatively amplified ATP and increased the sensitivity of a quantitative bioluminescence assay. However, it was difficult to increase t...
متن کاملContinuous-flow ATP amplification system for increasing the sensitivity of quantitative bioluminescence assay.
We constructed a novel ATP amplification reactor using a continuous-flow system, and this allowed us to increase the sensitivity of a quantitative bioluminescence assay by controlling the number of ATP amplification cycles. We previously developed a bioluminescence assay coupled with ATP amplification using a batch system. However, it was difficult to control the number of amplification cycles....
متن کاملAmplification ratio control system for copy number variation genotyping
We describe a generic design for ratiometric analysis suitable for determination of copy number variation (CNV) class of a gene. Following two initial sequence-specific PCR priming cycles, both ends of both amplicons (one test and one reference) in a duplex reaction, are all primed by the same universal primer (UP). Following each amplification denaturation step, the UP target and its reverse c...
متن کاملEffect of Lowering PCR Reaction Volumes and Increasing the Speed of DNA Amplification on the Sensitivity of PCR
ABSTRACT Recently, PCR is being reported more frequently with satisfactory results on the diagnosis of clinical infections. Widespread availability of PCR promises a sensitive and specific alternative, to traditional methods, but these benefits must be balanced against cost. Protocols using small volumes have described where the reaction taken place in capillary tubes, and small volumes work...
متن کاملMarkup cycles, dynamic misallocation, and amplification
We develop a tractable dynamic general equilibrium model of oligopolistic competition with a continuum of heterogeneous industries. Industries are exposed to aggregate and industry-specific productivity shocks. Firms in each industry set value-maximizing state-contingent markups, taking as given the behavior of all other industries. When consumers are risk-averse, industry markups are countercy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2009
ISSN: 0003-2697
DOI: 10.1016/j.ab.2009.08.025